急性梗阻性胰腺炎大鼠胰腺腺泡细胞凋亡的变化
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范志宁, 刘训良, 熊观瀛, 文 卫, 缪 林
急性梗阻性胰腺炎;胆胰管压力;腺泡细胞凋亡,范志宁,刘训良,熊观瀛,文卫,缪林,Changeofacinarcellapoptosisinratswith
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范志宁, 刘训良, 熊观瀛, 文卫, 缪林, 南京医科大学第一附属医院胰腺外科 江苏省南京市 210029
通讯作者: 范志宁, 210011, 江苏省南京市下关区姜家园121号, 南京医科大学第二附属医院消化内镜中心.
fanzhining@hotmail.com
电话: 025-58509932 传真: 025-58509931
收稿日期: 2006-01-17 接受日期: 2006-02-08
Change of acinar cell apoptosis in rats with acute pancreatitis induced by bile-pancreatic-duct obstruction
Zhi-Ning Fan, Xun-Liang Liu, Guan-Ying Xiong, Wei Wen, Lin Miao
Zhi-Ning Fan, Xun-Liang Liu, Guan-Ying Xiong, Wei Wen, Lin Miao, Department of Pancreatic Surgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210011, Jiangsu Province, China
Correspondence to: Dr. Zhi-Ning Fan, Center of Endoscopy, the Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, Jiangsu Province, China. fanzhining@hotmail.com
Received: 2006-01-17 Accepted: 2006-02-08
Abstract
AIM: To analyze the correlations of the degrees of acute pancreatitis with the duration of bile-pancreatic duct obstruction (BPDO) and acinar cell apoptosis by observing the change of bile duct pressure.
METHODS: The model of acute pancreatitis was established by ligation of bile-pancreatic duct. Bile duct pressure was examined 4, 8, and 12 h after BPDO as well as 1 and 3 d after removal of BPDO. Flow cytometry was used to detect the acinar cell apoptosis (AnnexinV-FITC/PI assay) and the expression of Caspase-3.
RESULTS: Compared with that in control group (16.42 ± 1.03), the pressure of bile-pancreatic duct increased with the prolonging of obstruction duration (49.98 ± 3.05, 90.20 ± 8.66, 589.00 ± 60.10 for 4, 8, 12 h after obstruction, respectively). When BPDO was removed, the pressure decreased and returned to the normal level within 1 d (17.50 ± 2.84, 16.37 ± 0.46 for 1 and 3 d after removal of obstruction, respectively), and the difference was significant (P < 0.01). AnnexinV-FITC/PI assay showed that the number of apoptotic cells increased significantly 4 h after BPDO, but it decreased while the number of dead cells increased 12 h after BPDO ......
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