重组蛋白rhTRAIL55-281与GST-rhTRAIL55-28
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刘洪波, 范学工, 黄建军, 李 宁, 应若素,
重组蛋白;rhTRAIL55-281;GST-rhTRAIL55-281;抗肿瘤药物;肝细胞;毒性;细胞凋亡,重组蛋白rhTRAIL55-281与GST-rhTRAIL55-281有肝细胞毒性,刘洪波
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刘洪波, 范学工, 李宁, 应若素, 彭建平, 中南大学湘雅医院感染病科 湖南省长沙市 410008
黄建军, 中南大学生物科学与技术学院生物化学系 湖南省长沙市 410008
刘洪波, 1992年湖南师大生物系学士, 讲师, 现在中南大学湘雅医院传染科攻读博士, 从事感染与免疫研究.
通讯作者: 范学工, 410008, 湖南省长沙市湘雅路87号, 中南大学湘雅医院感染病科. xgfan@hotmail.com
电话: 0731- 4327392
收稿日期: 2006-02-16 接受日期: 2006-02-21
Toxicity assay for recombinant protein of rhTRAIL55-281 and GST-rhTRAIL55-281 to hepatic cells
Hong-Bo Liu, Xue-Gong Fan, Jian-Jun Huang, Ning Li, Ruo-Su Ying, Jian-Ping Peng
Hong-Bo Liu, Xue-Gong Fan, Ning Li, Ruo-Su Ying, Jian-Ping Peng, Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha 410008, Hu’nan Province, China
Jian-Jun Huang, Department of Biochemistry, School of Biology Science and Technology, Central South University, Changsha 410008, Hu’nan Province, China
Correspondence to: Professor Xue-Gong Fan, Department of Infectious Diseases, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha 410008, Hu’nan Province, China. xgfan@hotmail.com
Received: 2006-02-16 Accepted: 2006-02-21
Abstract
AIM: To investigate the toxicity of recombinant protein rhTRAIL55-281 and GST-rhTRAIL55-281 to human hepatic cells, and to provide the experi-ment evidence for development of anti-tumor drugs.
METHODS: Primary cultured human hepatic cells were obtained from adult and fetus by trypsin digestion and mechanical separation. GST tag was cut off by Factor-Xa from recombinant protein GST-rhTRAIL55-281 to gain rhTRAIL55-281. Human hepatic cell strain L-02, primary cultured human hepatic cells from adult and fetus and normal human peripheral blood mononuclear cells (PBMCs) were intervened by recombinant protein rhTRAIL55-281 or GST-rhTRAIL55-281, and finally the apoptosis of cells were detected by flow cytometry.
RESULTS: The viability of primary cultured human hepatic cells from adult and fetus exceed 95%, and the purity of rhTRAIL55-281 without GST tag was 97% ......
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