玻璃化法冷冻保存对人胚胰岛的影响
http://www.100md.com
赵 松, 付英梅, 刘连新, 迟月明, 李秀芬
胰岛;玻璃化;冷冻保存,赵松,刘连新,付英梅,迟月明,李秀芬,通讯作者:,Cryopreservationofhumanfetalisletsbyvitrif
 |
| 第1页 |
参见附件(1008KB，5页)。
赵松, 刘连新, 哈尔滨医科大学第一临床医学院普通外科 黑龙江省哈尔滨市 150001
付英梅, 哈尔滨医科大学微生物教研室 黑龙江省哈尔滨市 150086
迟月明, 哈尔滨医科大学电镜中心 黑龙江省哈尔滨市 150086
李秀芬, 黑龙江省电力医院 黑龙江省哈尔滨市 150040
黑龙江省卫生厅资助项目, No. 2005-206
通讯作者: 刘连新, 150001, 哈尔滨市南岗区邮政街23号, 哈尔滨医科大学第一临床医学院普通外科. liulianxin@medmail.com.cn
电话:
收稿日期: 2006-02-20 接受日期: 2006-03-03
Cryopreservation of human fetal islets by vitrification
Song Zhao, Ying-Mei Fu, Lian-Xin Liu, Yue-Ming Chi, Xiu-Fen Li
Song Zhao, Lian-Xin Liu, Department of General Surgery, The First clinical college of Harbin Medical University, Harbin 150001, China
Ying-Mei Fu, Department of Medical Microbiology, Harbin Medical University, Harbin, 150086, China
Yue-Ming Chi, Electron Microscope Center, Harbin Medical University, Harbin, 150086, China
Xiu-Fen Li, Heilongjiang Power Hospital, Harbin, 150040, China
Correspondence to: Lian-Xin Liu, Department of General Surgery, The First clinical college of Harbin Medical University, Harbin 150001, China. liulianxin@medmail.com.cn
Received: 2006-02-20 Accepted: 2006-03-03
Abstract
AIM: To evaluate the efficiency of cryopre-servation of human fetal islets using vitrification as a potential feasible approach to islets bank establishment.
METHODS: Pancreatic islets of dead foetus aged 18 to 28 weeks induced by water bag were harvest by collagenase digestion. Islets were cultured and cryopreserved by computer programmed slow freezing and vitrification, respectively. The morphology and ultrastructure of thawed islets were studied in the two groups. Function of thawed islets was assessed by glucose-stimulated release of insulin, which was measured by radioimmunoassay. mRNA of insulin gene of the thawed islets was detected by using reversed-transcription polymerase chain reaction.
RESULTS: Morphologic studies of thawed islets demonstrated that both cryopreservation methods retained the integrity of islets ......
您现在查看是摘要介绍页，详见PDF附件(1008KB，5页)。