DNA损伤诱导胃癌细胞端粒酶活性和TRF2表达增高
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宁寒冰, 李继昌, 刘志国, 樊代明
端粒酶;端粒重复序列结合因子1;端粒重复序列结合因子2;胃癌;DNA损伤反应宁寒冰,李继昌,刘志国,樊代明.DNA损伤诱导胃癌细胞端粒酶活性和TRF2表达增高. 世界华人消化杂志2006;
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宁寒冰, 李继昌, 郑州大学第一附属医院消化内科 河南省郑州市 450052
刘志国, 樊代明, 国家肿瘤生物学重点实验室 第四军医大学西京医院全军消化病研究所 陕西省西安市 710032
宁寒冰, 1976年生. 郑州大学医学院博士生, 研究方向：消化道肿瘤.
国家自然科学基金资助项目, No.30400016
通讯作者: 刘志国, 710032, 陕西省西安市长乐西路15号, 第四军医大学西京医院消化病研究所. liuzhiguo @fmmu.edu.cn
电话: 029-84775229
收稿日期: 2006-01-03 接受日期: 2006-01-25
DNA damage increases telomerase activity and mRNA expression of telomeric repeat binding factor 2 in gastric cancer cells
Han-Bing Ning, Ji-Chang Li, Zhi-Guo Liu, Dai-Ming Fan
Han-Bing Ning, Ji-Chang Li, Department of Digestive Deseases, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China
Zhi-Guo Liu, Dai-Ming Fan, State Key Laboratory of Cancer Biology, Institute of Digestive Diseases, Xijing Hospital, the Fourth Military Medical University, Xi,an 710032, Shanxi Province, China
Supported by the National Natural Science Foundation of China, No. 30400016
Correspondence to: Zhi-Guo Liu, Institute of digestive disease, Xijing Hospital, Fourth Military Medical University, 15 West Changle Road, Xi’an 710032, Shaanxi Province, China. liuzhiguo @fmmu.edu.cn
Received: 2006-01-03 Accepted: 2006-01-25
Abstract
AIM: To investigate the possible involvement of telomerase and telomeric repeat binding factors (TRF1 and TRF2) in chemotherapeutic agents-induced DNA damage responses in gastric cancer cells.
METHODS: Gastric cancer cell line SGC7901 and MKN28 were treated with various concentrations of etoposide for 3, 6, 12, 24 and 36 h. Telomerase activity was measured by real-time quantitative telomeric repeat amplification protocol (RTQ-TRAP) assay. The expression of human telomerase reverse transcriptase (hTERT) mRNA was detected by real time reverse transcription polymerase chain reaction (RT-PCR). The expression of TRF1 and TRF2 were detected by Western blot and real time RT-PCR at protein and mRNA level, respectively ......
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