EGF中介的 NF-κB 及细胞外基质降解酶与胆管癌细胞侵袭相关性
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孔凡民, 刘小方, 李航宇, 隋春阳, 李昱骥,
表皮生长因子;基质金属蛋白酶;尿激酶型纤溶酶原激活剂;纤溶酶原激活物抑制剂;核转录因子-κB;胆管癌;侵袭孔凡民,刘小方,李航宇,隋春阳,李昱骥,张浩,郭仁宣.EGF中介的NF-kB及细胞外基质降解酶
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孔凡民, 李航宇, 李昱骥, 张浩, 郭仁宣, 中国医科大学附属第一医院普外科 辽宁省沈阳市 110001
刘小方, 青岛大学医学院附属烟台毓璜顶医院肝胆外科 山东省烟台市 264000
孔凡民, 1990年中国医科大学学士, 2001年博士, 副教授, 主要从事肝胆方面研究.
辽宁省教育厅高等学校科学研究项目 No. 05L510
通讯作者: 孔凡民, 110001, 辽宁省沈阳市, 中国医科大学附属第一医院普外科. kong_fanmin@yahoo.com.cn
电话: 024-83283336
收稿日期: 2006-01-10 接受日期: 2006-02-08
Correlations of epidermal growth factor-mediated nuclear factor-kappa B and matrix proteinase activity with invasions of cholangiocarcinoma cells
Fan-Min Kong, Xiao-Fang Liu, Hang-Yu Li, Chun-Yang Sui, Yu-Ji Li, Hao Zhang, Ren-Xuan Guo
Fan-Min Kong, Hang-Yu Li, Chun-Yang Sui, Yu-Ji Li, Hao Zhang, Ren-Xuan Guo, Department of General Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, China
Xiao-fang Liu, Department of Hepatobiliary Surgery, the Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Yantai 264000, Shandong Province, China
Supported by Scientific Research Project for higher education of Liaoning Province, No. 05L510
Correspondence to: Dr. Fan-Min Kong, Department of General Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001,China. kong_fanmin@yahoo.com.cn
Received: 2006-01-10 Accepted: 2006-02-08
Abstract
AIM: To explore the mechanism of epidermal growth factor (EGF) in the promotion of human cholangiocarcinoma cell invasion.
METHODS: Invasion assay and cell proliferation assay were performed in human cholangiocarcinoma cell line HuCCT1 treated with different concentrations of EGF. Reverse transcription polymerase chain reaction (RT-PCR) and Western blot were used to detect the expression of matrix metalloproteinases (MMPs), urokinase type plasminogen activator (uPA) and plasminogen activator inhibitor (PAI-1) in HuCCT1 cells. The activity of nuclear factor-kappa B (NF-κB) was determined by electrophoretic mobility shift assay (EMSA) ......
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