【摘要】 目的 观察表没食子儿茶素没食子酸酯(EGCG)对人胃癌细胞系(SGC-7901)裸鼠移植瘤生长及其血管生成的抑制作用，并探讨其分子机制。方法 采用人胃腺癌(SGC-7901)细胞裸鼠异种移植瘤模型，评价EGCG治疗人胃癌的有效性。间接免疫荧光标记流式细胞术检测移植瘤血管内皮生长因子(VEGF)的表达情况；免疫组化检测移植瘤中VEGF、基质金属蛋白酶-2(MMP-2)蛋白表达； Western blot分析不同浓度EGCG对细胞核转录因子NF-κB(P65)的表达情况。结果 间接免疫荧光标记的流式结果显示EGCG对VEGF的表达有浓度依赖性抑制作用。免疫组化VEGF和MMP-2蛋白的表达率与肿瘤侵入血管的程度有关；Western blot分析表明核转录因子NF-κB(P65)蛋白表达随EGCG浓度的升高而降低。结论 EGCG能抑制移植瘤新生血管形成，从而抑制裸鼠移植瘤的体内生长。

    【关键词】 EGCG；人胃腺癌SGC-7901细胞；裸鼠；VEGF； MMP-2

    Experimental study on VEGF expression and MMP-2 activation in transplanted tumor inhibited by EGCG in vivo

    CAO Juan, LUO Zhao-yang, ZHOU Wei,et al.

    Cancer Research Institute, Nanhua University, Hengyang, Hunan 421001,P.R.China

    【Abstract】 Objective To observe growth inhibition and expression level of vascular endothelial growth factor(VEGF)in tumor tissue transplanted with human gastric carcinoma cell line (SGC-7901)by epigallocatechin-3-gallate (EGCG)in vivo as well as approach molecule mechanism. Methods Antitumor effect of EGCG was determined using the xenografts models of human gastric carcinoma cell SGC-7901 in nude mice. The expression of VEGF in xenograft tumor cells was analyzed by indirect immunofluorescence. The expression of VEGF and matrix metalloproteinase-2 (MMP-2) in tumor tissues was examined by using immunohistochemical staining method. Protein expression of nuclear factor-KBP65(NF-κBP65) were observed by Western blot in different groups. Results The expression of VEGF in dose-dependant manner, which were observed by indirect immunofluorescence.Expression of VEGF and MMP-2 was higher in tumor tissues in relation to the extent of vascular invasion. Western blot analysis the expression of NF-κB(P65)protein in xenograft tumor is decreasing as adding the concentration of EGCG.Conclusion EGCG inhibits angiogenesis in human gastric carcinoma transplanted in mice so that growth of transplanted tumor can be inhibited in vivo. ......