[摘要]目的 探讨γ.射线辐射诱导白血病细胞凋亡的时程、剂量效应及其凋亡的发生机制。 方法 将对数生长的p53基因突变的Jurkat细胞经5、10、20Gy的γ.射线照射，其中一组在10Gy照射前加入Caspase抑制剂zVAD.fmk。照射后继续培养6、12、36、48h，收获细胞，用Annexin V标定、流式细胞术(FCM)定量分析细胞凋亡百分率。 结果 随培养时间的延长和照射剂量的增大，凋亡细胞数也增多。zVAD.fmk能抑制辐射诱导的细胞凋亡。 结论 凋亡细胞数量与照射后细胞培养时间及γ.射线的照射剂量呈线性关系，γ.射线诱导细胞凋亡可独立于p53基因，而Caspase在γ.射线诱导细胞凋亡中发挥重要作用。

    [关键词] 辐射;细胞凋亡;凋亡蛋白酶;白血病;Caspase抑制剂

    The time and dose kinetics of leukemic cells apoptosis induced byγ.radiation and the inhibiting effect of zVAD.fmk on the apoptosis

    LIU Jia-hao，WANG Wei，TANG Yue-hua，ZHAO Yu-xiang，WANG Chen-hu

    (Department of Pediatric，Zhongda Hospital，Southeast University，Nanjing210009，China)

    Abstract:Objective To investigate the dynamic process of leukemic cells apoptosis induced byγ.radiation and the inhibiting effect of zVAD.fmk on the apoptosis.Methods Jurkat cells，the p53gene of which had been mutated，were exposed toγ-radiation of5，10，20Gy，of which one group was added with zVAD.fmk before the ex-posure.The apoptosis cells were quantified by flow cytometry(FCM)after the cells were conjugated with FITC-An-nexin V.Results With the culture time prolonging and the dose of radiation increasing ......