[摘要]目的 探讨在实验条件下巨噬细胞集落刺激因子(M.CSF)在骨代谢过程中对破骨细胞的生物作用。 方法 用不同浓度的M.CSF和活化核因子κB的受体配体(RANKL)与鼠骨髓细胞培养，在加入或不加入牛骨片的情况下诱导产生破骨细胞。分别用抗酒石酸酸性磷酸酶(TRAP)染色法和骨小坑染色法检测TRAP阳性的破骨细胞量及破骨细胞功能。 结果 在一定范围内，M.CSF在RANKL存在的条件下诱导TRAP阳性破骨细胞成熟，并随M.CSF剂量的增加TRAP阳性细胞数明显增加(r=0.7504，P<0.01);且骨小坑数量也与M.CSF剂量呈明显正相关(r=0.9365，P≤0.0001)。缺乏M.CSF或RANKL均不能诱导TRAP阳性的破骨细胞产生(P=0.0191)。 结论 破骨细胞的增殖、分化及成熟是由M.CSF和RANKL共同调节的。M.CSF是破骨细胞代谢过程中的关键因子，具有促进破骨细胞对骨吸收的作用。M.CSF对骨代谢的主要作用之一是上调破骨系统。

    [关键词] 巨噬细胞集落刺激因子(M.CSF);骨骼;破骨细胞;抗酒石酸酸性磷酸酶染色;骨小坑染色

    The biologic effects of macrophage colony-stimulating factors on osteoclastic cells in vitro

    WU Xue-bin，Abe Etsuko

    (Department of Hematology，Beijing Railroad General Hospital，Beijing100038，China;Division of Endocrinology，Diabetes and Bone Disease，the Mount Sinai School of Medicine，NY10029，USA)

    Abstract:Objective To investigate the biologic effects of macrophage colony-stimulating factor(M.CSF)on osteoclastic cells during the bone metabolism in vitro.Methods The bone marrow cells of mice were treated with different concentration of M.CSF and RANKL in vitro.The mice stromal cells were cultured and osteoclast was sup-ported with or without bone slice.TRAP positive multinuclear cells were measured by tartrate-resistant acid phos-phatase(TRAP)staining and the function of osteoclast was detected by pit staining.Results The range of limiting M.CSF concentrations ......