survivin蛋白__(期刊论文)

Survivin在2株人脑胶质瘤细胞系中的表达

http://www.100md.com 《第四军医大学学报》 2005年第1期

survivin蛋白,,survivin蛋白；凋亡蛋白抑制因子；胶质瘤；反转录聚合酶链反应；免疫组化,0引言,1材料和方法,2结果,3讨论

     Expression of Survivin in two human brain glioma cell lines

    ZHEN HaiNing1, ZHANG Xiang1, WANG XiLing1, FU LuoAn1, LIANG JingWen1, HU PeiZhen2, MA FuCheng2, GUO Yan1, LIN Wei1

    1Institute of Neurosurgery of Chinese PLA, Xijing Hospital, 2Department of Pathology, School of Basic Medicine, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To study the expression levels of mRNA and protein of inhibitor of apoptosis protein, survivin, in human brain glioma cell lines BT325 and SHG44 and its significance. METHODS: The expression levels of mRNA and protein of survivin were investigated respectively by reverse transcription polymerase chain reaction (RTPCR) and immunohistochemistry methods in cell lines BT325 and SHG44. RESULTS: The high expression of mRNA and protein of survivin, as well as low expression of splice variant of survivin, survivin2B, was observed in BT325 cells. Protein of survivin was located in cytoplasm of BT325 cells, but mRNA and protein of survivin were not expressed in SHG44 cells. CONCLUSION: Survivin plays an important role in maintaining the biological features of BT325 cells. BT325 and SHG44 cells can be used as ideal cell models respectively for research of gene inhibition and gene transfer of survivin. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/04/12/96/52/24.htm

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