β干扰素__(期刊论文)

人IFNβ基因脂质体转染胶质瘤细胞对凋亡的诱导作用

http://www.100md.com 《第四军医大学学报》 2005年第1期

β干扰素,,β干扰素；脂质体；转染；胶质瘤细胞；凋亡,0引言,1材料和方法,2结果,3讨论

     Induction of apoptosis of glioma cell line SHG44 by transfection of human interferonβ gene liposome

    LIN Wei, ZHANG Xiang, WANG ZhanXiang, FEI Zhou, ZHANG JianNing, FU LuoAn, LIU WeiPing, GUO Yan, WANG XiLing, LIANG JingWen

    Chinese PLA Institute of Neurosurgery，Xijing Hospital，Fourth Military Medical University，Xian 710033，China

    【Abstract】 AIM: To investigate the transfection of liposome containing pSV2IFNβ and its effects on the induction of apoptosis of human glioma cell line SHG44. METHODS: MTT assay was used to measure the proliferation of SHG44 cells transfected by human interferonβ liposome containing pSV2IFNβ and the IFNβ expression in SHG44 cells was observed by indirect immunofluorescence staining. FCM Annexin was used to detect the apoptotic SHG44 cells after transfection with pSV2IFNβ and the morphological changes of SHG44 cells were detected by electron microscopy. RESULTS: The proliferation of SHG44 was obviously inhibited after transfection of human interferonβ liposome containing pSV2IFNβ and the inhibition rates were 16.7% and 32.7% respectively on d 4 and d 6 after transfection. The results of indirect immunofluorescence staining showed that the IFNβ expressions of SHG44 cells were obvious after transfection and the results of FCM showed that the apoptotic cell rate of the transfection group and control group were 66.8% and 19.8%, respectively. Significant differences were found between the two groups (P<0.01). Morphological changes of apoptosis were also found by electron microscopy. CONCLUSION: Human interferonβ gene liposome containing pSV2IFNβ can transfect human glioma cell line SHG44 and significantly induce the apoptosis of cells line SHG44. ......

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