【摘要】 目的 建立鳖甲山楂颗粒中熊果酸含量测定的方法。方法 高效液相色谱法。色谱柱：Zorbax SB-C18(4.6mm×2.50mm，5μm)；流动相：甲醇-水(0.5%乙酸胺)(84：16)；流速：1ml/min；检测波长：215nm；柱温：30℃。结果 熊果酸线性范围0.5548～2.7740μl，平均回收率为99.53%，RSD为1.65%。结论 该方法准确，可靠，可用于控制鳖甲山楂颗粒的质量。

    【关键词】 熊果酸；高效液相；含量测定

    【Abstract】 Objective To establish the method to determine the content of urolic acid in micro-porphyrizaed bie-zha granula.Methods High performance liquid chromatography was used.C18 column was used;the mobile phase was consisted of MeOH-warter(84:16);the detection wavelength was at 215nm;the flow speed was 1ml/min;the column temperature was 30℃.Results The HPLC recoveries was 99.53% for urolic acid with RSD=1.65%.Conclusion The method can be used for controlling the quality of the micro-porphyrizaed granula.

    【Key words】 urolic acid;HPLC;content determination

    鳖甲山楂颗粒剂由鳖甲和山楂两味药组成 ......