Effects of Radix Astragali injection on expressions of glial fibrillary acidic protein in periª²intracerebral hemorrhage foci

    ZHANG Shouª²Xin1, WANG Bingª²Ang2, XIAO Wen1, LI Gaiª²Li1, ZHANG Hongª²Bin1

    1Department of Neurology, 2Department of Orthopaedics, Third Hospital of Chinese PLA, Baoji 721004, China

    ¡¾Abstract¡¿ AIM: To study the effects of Radix Astragali injection on glial reaction in and around cerebral hemorrhagic area. METHODS: Collagenase was injected into the stratum of the rat to produce a bleeding. Immunohistochemical ABC method was used to study the expressions of glial fibrillary acidic protein (GFAP), a marker for astrocyte, in the rat brain at different time points in Radix Astragali group and control group after intracerebral hemorrhage. RESULTS: There were more densely distributed GFAPª²immunoreactive cells (398¡À6)/mm2 around the blood clot 12 h after collagenase injection and the number of GFAPª²positive cells was decreased by Radix Astragali [(368¡À3)/mm2, P<0.05]. After 72 h, GFAPª²positive cell attenuated (342¡À5)/mm2 and was enhanced by Radix Astragali [(352¡À4)/mm2, P<0.05]. CONCLUSION: The regulatory effect of Radix Astragali on astrocytes may be one of its mechanisms in repairing nervous functions after cerebral hemorrhage. ......