Role of tumor necrosis factorª²related apoptosisª²inducing ligand(TRAIL)and its receptors in subarachnoid hemorrhage inducing apoptosis in vasospasm artery endothelial cells

    WANG Liang1£¬GAO Guoª²Dong1£¬ZHAO Zhengª²Wei1£¬RAO Zhiª²Ren2, QIN Huaiª²Zhou1, JIA Dong1, SHEN Jing2

    1Department of Neurosurgery, Tangdu Hospital, Fourth Military Medical University, Xi¬ðan 710038, China ,2Department of Neurobiology, School of Basic Medicine,Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To study the role of tumorª²related apoptosisª²inducing ligand(TRAIL) and its receporª²DR4 , receporª²DR5 in apoptotic endothelial cells of vasospasm artery after subarachnoid hemorrhage. METHODS: Fiftyª²four rabbits were used in the present study. The population was randomly divided into three groups: control group, subarachnoid hemorrhage(SAH) group and sham operated group and the groups were sacrificed respectively on preª²operation and 0, 2, 4 and 7 d (6 per group). The SAH models were made by doubleª²hemorrhage method. Cerebral vasospasm (CVS) was confirmed by angiogram. TRAIL protein and its receptor DR4 ,DR5 expression positive number and apoptotic cell number were determined using immunohistochemical technique and TdTª²mediated dUTPª²biotin nick end labeling (TUNEL). RESULTS: Angiographic vasospasm began on 0 d and peaked on 2 d to 4 d. In TUNEL studies, control rabbits did not demonstrate apoptosis in endothelial cells of the basilar arteries. CVS rabbits beginning on 0 d, apoptosis were noted in endothelial cells(P<0ª±01).In immunohistochemical study, TRAIL ......