【摘要】 目的 通过截除幼年大鼠双前肢建立颈椎间盘退变的动物模型。方法 健康4周龄雄性SD大鼠76只，随机分为两组，手术组40只，对照组36只。手术组动物麻醉后于双上肢根部截除肢体，分别于术后3、6、9、12个月随机分为4组，每组10只处死大鼠后摄颈椎正侧位X线片，并切取椎体标本制备C2～C3至T2～T3的中矢状面组织学切片，分别进行HE、番红O染色行椎间盘组织学观察。对照组动物分别于实验开始后4、8、12、16个月分为4组进行与实验组相同的处理。结果 截除双前肢后，双后肢大鼠全部存活，实验组大鼠术后9、12个月均出现颈椎间盘退变的X线征象，光镜检查证实其椎间盘均发生了严重的退变，脊索性髓核完全为纤维软骨性髓核取代，软骨终板缺损或消失，基质中胶原纤维排列紊乱，椎间盘突出并骨化。而正常大鼠的椎间盘仅发生轻度退变。结论 本造模方法不损伤动物靶器官正常的解剖结构，成功率高、重复性好，符合人类颈椎间盘退变规律。

    【关键词】 疾病模型；动物；颈椎间盘；X线摄片；组织学

    The establishment of degenerative cervical disc animal model in bipedal rats

    WANG Wei-ming，JIN Da-di，QU Dong-bin，et al.

    Department of Orthopaedics,Affiliated Zhongshan Hospital of Dalian University，Dalian 116001，China

    【Abstract】 Objective To develop a scientific and reproducible degenerative disc animal model for the study on the cervical disc.Methods The bipedal rats model was developed in 40 infancy SD rats by forelimb amputation.Thirty-six normal rats in the same age served as the control.When the rats in the experimental group were 3,6,9 and 12 months (named E3，E6，E9 and E12 group respectively) and in the control group were 4 ......