Effect of aprotinin on lung ischemia/reperfusion injury in lung transplantation in canines

    TIAN Feng, CHENG Qingª²Shu, LI Xiaoª²Fei, WANG Xiaoª²Ping, JIANG Tao, ZHOU Yongª²An, YANG Ye, CHEN Lianª²Hong

    Department of Thoracic Surgery,Tangdu Hospital,Fourth Military Medical University, Xi¬ðan 710038, China

    ¡¾Abstract¡¿ AIM£º To evaluate the effect of aprotinin on the ischemia/reperfusion injury of the lung in lung transplantation in canines. METHODS: Twenty male canines were randomly divided into two groups. In each group the canines were randomly assigned to donors (n=5) and recipients (n=5). The recipients received either aprotinin 25 U/kg iv. infusion (Group I) or equivalent volumes of 50 g/L glucose i.v.(Group II) before reperfusion. The lung tissue samples were obtained one hour after reperfusion. The pulmonary water content, tissue myeloperoxidase (MPO) activity, content of MDA and nitric oxide (NO) were measured. Microscopic examination of lungs was also conducted. RESULTS: The pulmonary water content (73ª±65¡À4ª±31)%, the tissue MPO activity (0ª±21¡À0ª±05 ¦¤OD/min¡¤g), the content of MDA (0ª±17¡À0ª±004 nmol/mg¡¤prot) and the content of NO (12ª±60¡À8ª±73 nmol/mg¡¤prot) in group I were significantly lower respectively than those in control (81ª±33¡À3ª±67)%, (0ª±37¡À0ª±10 ¦¤OD/min¡¤g), (0ª±29¡À0ª±010 nmol/mg¡¤prot) and (35ª±09¡À13ª±73 nmol/g¡¤prot) (P<0ª±05). The microscopic examination showed that there were severe leukocyte infiltration and edema formation in the alveolar space in group II, but the changes were less severe in group I. CONCLUSION: The application of aprotinin before reperfusion can protect the lung against ischemia/reperfusion injury in lung transplantation in canine. ......