【摘要】 目的 观察甲状腺素对SD大鼠胰岛B细胞凋亡核苷酸、Caspase-3、Bcl-2的影响，以阐明甲状腺功能亢进时合并糖尿病或葡萄糖耐量降低的可能病理机制。方法 末端标记dUTP(TUNEL)法观察SD大鼠胰岛B细胞凋亡核苷酸、免疫组织化学观察Caspase-3和Bcl-2的表达阳性率变化。结果 (1)小剂量甲状腺素对在体SD大鼠胰岛B细胞凋亡核苷酸、Caspase-3表达阳性率明显高于对照组(P<0.01)，而Bcl-2的表达率明显低于对照组(P<0.01)，具有显著的诱导大鼠胰岛B细胞凋亡的作用；(2)同时甲状腺素喂饲后的大鼠胰腺腺泡细胞明显发生脂肪变性。结论 甲状腺素对在体大鼠胰岛B细胞具有明显诱导凋亡的作用，此作用在甲状腺功能亢进时对糖的代谢异常具有重要作用，可能是甲状腺功能亢进时合并糖尿病及糖耐量异常的重要的病理机制。

    【关键词】 甲状腺素；B细胞；凋亡；TUNEL；Caspase-3

    The effects of thyroxin to the apoptosis of pancreatic β cells in rats

    CHEN Kuan-lin,FANG Ming-ming,SUN Guo-qing,et al.Clinical Department，Jiangsu Staff’s Medical University,Nanjing 210029,China

    【Abstract】 Objective Patients with hyperthyroidism are mostly suffering from diabetes mellitus or impaired glucose tolerance，but the mechanism is uncertain by now，in order to research this we studied the role of low dosage of thyroxin to the apoptosis of pancreatic β cells in rats.Methods Observing the apoptotic nucleosides with terminal UTP nick end labelling (TUNEL), Caspase-3 and Bcl-2 with immunochemo histology of pancreatic β cells in rats.Results (1) Low dosage of thyroxin has the significant role to the apoptosis of pancreatic β cells in rats ......