蛋白_____内皮抑素；硫氧还蛋白融合表达系统；融合蛋白

硫氧还蛋白融合表达系统表达的小鼠内皮抑素之纯化和生物学活性鉴定

http://www.100md.com 《第四军医大学学报》 2006年第5期

蛋白,,内皮抑素；硫氧还蛋白融合表达系统；融合蛋白,0引言,1材料和方法,2结果,【参考文献】

     Purification of mouse endostatin in thioredoxin fusion expression system and identification of its biological activity

    MA WeiLie，LIU Fang，HE ChengWei，HE ZhenHui

    Department of Biochemistry，Guangdong Medical College，Zhanjiang 524023，China

    【Abstract】 AIM：To purify mouse endostatin by using thioredoxin fusion expression system and to identify its biological activity. METHODS: The pThioHisendo recombinant plasmid was transformed into BL21. After induction with IPTG, thioredoxinendo fusion protein was expressed in BL21 and the product was identified as inclusion body by SDSPAGE. The expression product was purified by affinity chromatography through Nicolumn. The purified protein was detected by chicken chorioallantoic membrane(CAM) angiogenesis inhibitory assay and endothelial cell proliferation inhibitory assay. RESULTS: High purity thioredoxinendostatin fusion protein was obtained by SDSPAGE analysis. The purity of the protein was over 95%. The protein possessed the inhibitory activity of endothelial cell proliferation and inhibited the angiogenesis of CAM [inhibitory rate: (49.6±4.1)%, (53.2±2.3)%, (55.0±3.6)% and (67.1±5.7)%]. CONCLUSION: The mouse endostatin recombinant fusion protein expressed in E.coli by using thioredoxin fusion expression system is easy to be purified and possesses high activity. ......

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