胚胎培养_____体外受精;_胚胎培养;

胚胎培养液和体外操作环境对IVFET妊娠率的影响

http://www.100md.com 《新疆医科大学学报》 2006年第2期

胚胎培养,,体外受精;,胚胎培养;,妊娠,1资料与方法,2结果,3讨论,参考文献：

     摘要: 目的: 探讨胚胎培养液和体外操作环境对体外受精胚胎移植(IVFET)妊娠率的影响，以便建立更适宜的胚胎体外培养体系。方法: 对78例(79个周期)不孕症患者的临床资料进行回顾性分析。79个IVFET周期患者分为2组，Ⅰ组21个周期使用单纯EBSS培养液,无37℃恒温、保湿、维持pH的设施,Ⅱ组58个周期使用HTF、IVCONE序列培养基，且配备37℃恒温、保湿、维持pH恒定的设施，比较2组的受精率、卵裂率、优质胚胎率、着床率和临床妊娠率。结果: Ⅰ组与Ⅱ组的受精率、卵裂率、优质胚胎率分别为74.88%、76.39%，72.46%、76.01%，38.00%、41.86%，差异均无统计学意义(P>0.05)，而胚胎着床率、临床妊娠率分别为2.32%、19.75%， 9.52%、44.82%，Ⅱ组显著高于Ⅰ组，差异有统计学意义(P<0.01)。结论: 序列培养基与37℃恒温、保湿、维持pH设施结合应用，改善了胚胎的潜在质量，显著提高了IVF的妊娠率。

    关键词: 体外受精; 胚胎培养; 妊娠

    Influence of embryos culture environment on the pregnancy rate

    HU Bo, Mayire·Tuerxun, SHI Min, et al

    (Department of Reproductive Medicine, First Affiliated Hospital, Xinjiang Medical University, Urumuqi 830054, China)

    Abstract: Objective: To investigated the Influence in vitro embryos culture environment on the pregnancy rate so that to establish the more suitable culture system of embryos. Methods: Two culture media were used in two groups. The first was HTF and IVCONE media culture system (maintaining 37℃, relative humidity, 5%CO2 ) which was used in 58 IVFET cycles, and the second was EBSS media system (not maintaining 37℃, relative humidity, 5%CO2 ) in 21 cycles. The fertilization rate, cleavage rate, good embryo rate, embryo imbed rate and pregnancy rate were compared between these two groups. Results: There were significantly higher embryo imbed rate and pregnancy rate (19.75%, 44.82% respectively) used HTF and IVCONE media culture system group as compared those in EBSS media system group (2.32%, 9.52% respectively). There were no significant differences in fertilization rate, cleavage rate, good embryo rate between two groups (76.39%, 76.01%, 41.86% and 74.88%, 72.46%, 38% respectively). Conclusion: HTF and IVCONE media culture system can improve the pregnancy rate significantly. ......

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