Study of estrogen receptors in the lung of male rats

    LIN Yong，SUN Si-qing，ZHU Xiao-li，ZHANG Qiang

    (Department of Respiratory，Zhongda Hospital，Southeast University，Nanjing210009，China)

    Abstract:Objective To investigate the expression and distribution of the estrogen receptors(ER)in the lung of male rats.Methods The expressions of ER.αmRNA and ER.βmRNA in lung tissues was detected by reverse transcription-poly-merase chain reaction(RT-PCR)，and ER.βprotein in lung was detected by immunohistochemistry.Results Aside from weak expression of ER.αmRNA，lung tissue showed moderate expression of ER.βmRNA.The positive ER.βimmunoreac-tivity was presented in columnar epithelium，intermediate cells，basal cells，smooth muscle cells of lung tissue and in pulmo-nary vascular smooth muscle cells，endothelial cells.Conclusion ER.βis distributed in lung tissue of male rats.

    Key words:estrogen receptor;lung tissue;immunohistochemistry;rats

    [摘要]目的: 探讨大鼠孤束核(NTS)内组胺H3 受体对哮喘发作时大脑Fos蛋白表达的影响及其意义。 方法: 取健康雄性SD大鼠制备哮喘模型并诱发哮喘发作，采用中枢立体定位及微量注射技术，分3组分别在NTS内微量注射无菌生理盐水、H3 受体激动剂R.α.methylhistamine(RMHA)以及H3 受体拮抗剂thioperamide(Thio)加RMHA ......