ffect of Angiotensinª²(1ª²7) on cell proliferation and collagen synthesis of cardiac fibroblasts induced by vasopressin

    NIU Xiaoª²Lin, ZHAO Lianª²You, ZHENG Qiangª²Sun, HUANG Zhiª²Gang, WANG Xianª²Mei, WU Liª²Jun, SHANG Fuª²Jun

    Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi¬ðan 710038, China

    ¡¾Abstract¡¿ AIM: To investigate the effect of Angiotensinª²(1ª²7) [Angª²(1ª²7)] on the cell proliferation and collagen synthesis of cardiac fibroblasts (CFs) induced by vasopressin(AVP). METHODS: CFs were isolated and cultured from neonatal SD rats. Cell number and cell cycle were evaluated by MTT assay and flow cytometry technique and Type ¢ñand ¢ó collagen mRNA expressions in CFs were analyzed by RTª²PCR. RESULTS: ¢Ù 0.1 ¦Ìmol/L AVP significantly increased absorption value of CFs (0.24¡À0.01) compared with that in control group (0.14¡À0.01, P<0.01), and 0.001-1 ¦Ìmol/L Angª²(1ª²7) attenuated the absorption value of AVP group in a concentration dependent manner (0.22¡À0.01, 0.21¡À0.01, 0.18¡À0.01 and 0.16¡À0.01, P<0.01). ¢Ú Compared with those in control group (5.40¡À0.67, 10.82¡À2.40), cells in S stage (14.00 ¡À0.94) and proliferation index (23.44¡À1.75) markedly increased in 0.1 ¦Ìmol/L AVP group (P<0.01). In the presence of 0.1 ¦Ìmol/L Angª²(1ª²7), cells in S stage (8.52¡À0.70) and the proliferation index (16.24¡À2.04) were significantly lower than those in AVP group (P<0.01). ¢Û mRNA expressions of type¢ñand ¢ó collagens in AVP group were significantly higher than those in control group (P<0.01) and in the presence of 0.001-1 ¦Ìmol/L Angª²(1ª²7), mRNA expressions were reduced in a concentration dependent manner (P<0.05). CONCLUSION: Angª²(1ª²7) inhibits the proliferation of CFs and the synthesis of type ¢ñand ¢ó collagens. ......