熟地黄_____熟地黄；反相高效液相色谱法；5羟甲基糠醛

RPHPLC法测定熟地黄中5羟甲基糠醛的含量

http://www.100md.com 《第四军医大学学报》 2006年第6期

熟地黄,,熟地黄；反相高效液相色谱法；5羟甲基糠醛,0引言,1材料和方法,2结果,3讨论,【参考文献】

     RPHPLC determination of 5hydroxymethylfurfural in prepared rehmannia root

    QIN XiangYang, ZHOU Jun, LI XiaoYe, SUN XiaoLi

    Department of Chemistry, School of Pharmacy, Fourth Military Medical University，Xian 710033，China

    【Abstract】 AIM: To develop a HPLC method for the determination of 5hydroxymethylfurfural (5HMF) in prepared rehmannia root. METHODS: The RPHPLC method was used. The analytical column was a ZODBAXC18(4.6 mm×150 mm, 5 μm) and the mobile phase consisted of methanol 0.01 mol/L phosphoric acid aqueous solution (70∶30). The wavelength was set at 284 nm with the Detector and the flowrate 0.8 mL/min, the column temperature 25℃ and the injection volume 20 μL. RESULTS: Over the range of 0.44-2.18 μg, a good linear relation existed between 5HMF and the peak area (r=0.999 9, n=5). The average recovery of the added sample was 99.0% and RSD 0.8% (n=6). CONCLUSION: The proposed method can be used for quantitatively analyzing 5HMF in a convenient and economic way, with high accuracy and good reproducibility. This method for the determination of 5HMF in prepared rehmannia root can be popularized. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/04/12/96/67/41.htm

您现在查看是摘要页，全文长 6330 字符。