间接ELISA法对H pylori免疫牛初乳中抗体的检测
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周冬仁, 王 锋, 张常印, 崔 山, 唐泰山,
间接ELISA;幽门螺杆菌;初乳周冬仁,王锋,张常印,崔山,唐泰山,偶健.间接ELISA法对Hpylori免疫牛初乳中抗体的检测. 世界华人消化杂志2006;14(11)1103-1106
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周冬仁, 王锋, 崔山, 偶健, 南京农业大学动物科技学院 江苏省南京市 210095
张常印, 唐泰山, 江苏省进出境检验检疫局动检实验室 江苏省南京市 210001
江苏省国际合作项目, No. BZ2005041
通讯作者: 王锋，210095，江苏省南京市，南京农业大学动物科技学院. f_wang2001@sina.com
收稿日期: 2006-01-10 接受日期: 2006-02-09
Indirect ELISA in detection of antibodies in colostrums of the cows immunized with Helicobacter pylori
Dong-Ren Zhou, Feng Wang, Chang-Yin Zhang, Shan Cui, Tai-Shan Tang, Jian Ou
Dong-Ren Zhou, Feng Wang, Shan Cui, Jian Ou,College of Animal Science and Technology, Nanjing Agriculture University, Nanjing 210095, Jiangsu Province, China
Chang-Yin Zhang, Tai-Shan Tang, Laboratory of Animal Quarantine, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 210001, Jiangsu Province, China
Supported by the International Cooperation Project of Jiangsu Province, No. BZ2005041
Correspondence to: Feng Wang, College of Animal Science and Technology, Nanjing Agriculture University, Nanjing 210095, Jiangsu Province, China. f_wang2001@sina.com
Received: 2006-01-10 Accepted: 2006-02-09
Abstract
AIM: To observe the changes of specific antibodies in the cow colostrums by indirect enzyme linked immunosorbent assay (ELISA).
METHODS: Two pregnant cows (non-parous and parous) were immunized with H pylori whole-cell antigen (4×109 CFU) via intramuscular injection in the neck. The colostrums were collected with 7 days after parturition.
RESULTS: H pylori induced significant immune responses in both cows, and the efficacy of anti-H pylori in the parous cow reached the peak values (5.84) at the first day after parturition, which was significantly higher than that in the non-parous cows (3.51, P < 0.05). However, the efficacies dropped quickly with 7 days. Weak positive anti-H pylori were observed only at the first day in the control cow.
CONCLUSION: The immune response is superior in the parous cow to that in the non-parous one ......
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