流体动力学法乙型肝炎病毒感染动物模型的建立
http://www.100md.com
应若素, 范学工, 李 宁, 朱 才, 刘洪波
流体动力学;乙型肝炎病毒;动物模型,应若素,范学工,李宁,朱才,刘洪波,应若素,通讯作者:,电话:,收稿日期:,接受日期:,Developmentofa
 |
| 第2页 |
 |
| 第1页 |
参见附件。
应若素, 范学工, 李宁, 朱才, 刘洪波, 中南大学湘雅医院感染病科 湖南省长沙市 410008
应若素, 2000年苏州大学医学院学士, 2003年中南大学湘雅医学院硕士, 现为中南大学湘雅医院在读博士, 主要从事病毒性肝炎的研究.
湖南省科技厅科技计划重点资助项目, No. 05SK2001
通讯作者: 范学工, 410008, 湖南省长沙市湘雅路87号, 中南大学湘雅医院感染病科. xgfan@hotmail.com
电话: 0731-4327392 传真: 0731-4327332
收稿日期: 2006-02-21 接受日期: 2006-03-11
Development of a hepatitis B virus infection animal model by hydrodynamics
Ruo-Su Ying, Xue-Gong Fan, Ning Li, Cai Zhu, Hong-Bo Liu
Ruo-Su Ying, Xue-Gong Fan, Ning Li, Cai Zhu, Hong-Bo Liu, Department of Infectious Diseases, Xiangya Hospital of Central South University, Changsha 410008, Hunan Province, China
Supported by the Research Foundation of Science and Technology of Hunan Province, No. 05SK2001
Correspondence to: Dr. Xue-Gong Fan, Department of Infectious Diseases, Xiangya Hospital of Central South University, 87 Xiangya Road, Changsha 410008, Hunan Province, China. xgfan@hotmail.com
Received: 2006-02-21 Accepted: 2006-03-11
AbstractAIM: To develop a simple, convenient, efficient and stable animal model of hepatitis B virus (HBV) infection, and observe the dynamic changes of viral replication as well as expression in the model.
METHODS: pcDNA3.1-HBV was delivered into BALB/c mice using the hydrodynamic tail vein injection method. In a week, the levels of HBsAg, HBeAg, anti-HBs, anti-HBe, and anti-HBc were determined with time-resolved immunofluorometric assay (IFMA) kit, and the titers of HBV DNA were analyzed by fluorogenic quantitative polymerase chain reaction (FQ-PCR). In addition, viral specific proteins (HBsAg and HBcAg) in the liver were assayed by immunohistochemical staining.
RESULTS: A mouse model of acute HBV infection was developed successfully. HBsAg expression reached the peak on day 1, and dropped gradually. The level of HBeAg was low, and the level of anti-HBc, anti-HBe, and anti-HBs were first detected on day 4, 5 and 7, respectively ......
您现在查看是摘要介绍页，详见PDF附件。