扑热息痛引起肝细胞损害的机制
http://www.100md.com
田 丰, 李 岩, 王 颖, 吴作艳, 王学清
扑热息痛;肝损害;坏死;细胞凋亡田丰,李岩,王颖,吴作艳,王学清.扑热息痛引起肝细胞损害的机制. 世界华人消化杂志2006;14(11)1107-1110,田
 |
| 第1页 |
参见附件(1503KB，4页)。
田丰, 李岩, 王颖, 吴作艳, 王学清, 中国医科大学附属第二医院(盛京医院)消化科 辽宁省沈阳市 110004
通讯作者: 李岩, 110004, 辽宁省沈阳市和平区三好街36号, 中国医科大学附属第二医院(盛京医院)消化科. liyan99@medmail.com.cn
电话: 024-83956416
收稿日期: 2006-02-13 接受日期: 2006-03-07
Mechanism of acetaminophen-induced hepatocellular injury
Feng Tian, Yan Li, Ying Wang, Zuo-Yan Wu, Xue-Qing Wang
Feng Tian, Yan Li, Ying Wang, Zuo-Yan Wu, Xue-Qing Wang, Department of Gastroenterology, the Second Affiliated Hospital of Chinese Medical University, Shenyang 110004, Liaoning Province, China
Correspondence to: Yan Li, Department of Gastroenterology, the Second Affiliated Hospital of Chinese Medical University, Shenyang 110004, Liaoning Province, China. liyan99@medmail.com.cn
Received: 2006-02-13 Accepted: 2006-03-07
Abstract
AIM: To investigate the role of hepatocellular apoptosis in hepatitis induced by acetaminophen (AAP).
METHODS: The Sprague Dawley (SD) rat model of acetaminophen-induced hepatitis was established using AAP. After administration, the rats were killed at the end of 3, 6, 12, and 24 h, respectively. In both AAP and control group, we detected levels of serum alanine transaminase (ALT), observed pathological changes of hematoxylin and eosin (HE) stained liver with light microscope, and detected hepatocellular apoptosis using TUNEL method and electron microscopy.
RESULTS: The level of serum ALT (nkat/L) in AAP group was significantly higher than that in control group (3, 6, 12, 24 h: 1167 ± 151 vs 587 ± 89, 2154 ± 255 vs 573 ± 76, 4221 ± 929 vs 751 ± 82, 13 203 ± 1393 vs 780 ± 161, all P < 0.01), which increased progressively and reached the peak at 24 h. In AAP group, different degrees of necrosis was detected and aggravated progressively, and it also reached the peak at 24 h. There were a large number of apoptotic hepatocytes around central veins and they reached the peak at 12 h in AAP group. However, few apoptotic hepatocytes were observed in control group ......
您现在查看是摘要介绍页，详见PDF附件(1503KB，4页)。