【摘要】 目的 观察不同浓度的视黄酸(retinoid acid,RA)对人乳腺癌细胞系MCF-7细胞增殖及细胞周期的影响。方法 以不同浓度RA(10-10～10-6mol/L)处理细胞，采用活细胞计数法观察细胞增殖情况，用氨基安替比林法测定碱性磷酸酶(AKP)活性；通过流式细胞仪技术，测定RA作用后其细胞周期分布。结果 RA对MCF-7细胞的增殖有明显抑制作用，10-7mol/L RA作用5天后活细胞计数为对照组的60%，细胞在G0/G1期停滞；RA还可使MCF-7细胞AKP活性增高，这种作用具有时间和剂量依赖性。结论 RA对MCF-7细胞有明显的增殖抑制作用，细胞在G0/G1期停滞。

    【关键词】 视黄酸；碱性磷酸酶；细胞周期；乳腺肿瘤

    Effects of RA on proliferation and cell cycle of MCF-7 in vitro

    HUI Ning,XU Ming-juan,LIU An,et al.Department of Obstetrics and Gynecology,Changhai Hospital,The Second Military Medical University,Shanghai 200433,China

    【Abstract】 Objective To study the effects of RA(Retinoid Acid),a synthetic RA,on the cell proliferation and cell cycle of a human breast carcinoma cells.Methods After treated with different concentration of RA(10-10～10-6mol/L),alkaline phosphatase(AKP) activity was determined.The cell cycle progression was examined with flow cytometry.Results RA inhibited the proliferation of MCF-7 cells,the cell number was 60% compared with the control after the treatment with RA(10-7mol/L) for 5 days.AKP activity craised significantly after treatment with RA(10-6mol/L)for 1～5days.Dose-response studies revealed that the AKP activity of MCF-7 cells increased progressively as a function of RA concentrations.Conclusion Glucocortocoids play an important role in the regulation of MCF-7 cell proliferation and differentiation,inducing G0/G1 arrest. ......