exosomes__(期刊论文)

K562细胞分泌的exosome的免疫电镜研究

http://www.100md.com 《第四军医大学学报》 2005年第5期

exosomes,,exosomes；K562细胞；超滤；免疫电镜,1材料和方法,2结果,3讨论,【参考文献】

     Observation of K562 cellsecreted exosomes with immunoelectron microscope

    BU Ning1, SUN BingZhong1, LI QiLing2，YU YueCheng2，ZHANG Tao1，WANG YiWei1， QIAO QingDa1

    1Department of Hematology, 2Department of Obstetrics and Gynecology，Xijing Hospital, Fourth Medical University, Xian 710033, China

    【Abstract】 AIM: To observe K562 cellsecreted exosomes under immunoelectron microscope and to discuss their potential clinical value. METHODS: Exosomes were purified by ultrafiltration centrifugation and sucrose gradient ultracentrifugation. Electron microscopy was used to observe their ultrastructures. The expression of HSP70, ICAM1 and ABL were investigated by solid phase immunoelectron microscopic method (SPIEM). RESULTS: Transmission electron microscopy revealed that the exosomes from K562 cell culture supernatants were 50 to 100 nm membrane vesicles. They were round or ellipse in shape. Cavum had low electrondensity components, which were labeled by colloidal gold HSP70, ICAM1 and ABL. Immune electron microscopy showed the deposition of electron particles in the extramembrane and cavum of the vesicles. CONCLUSION: Ultrafiltration centrifugation and sucrose gradient ultracentrifugation can be used to purify K562 cellssecreted exosomes. The exosomes express HSP70, ICAM1 and ABL protein and have immunogenicity. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/04/25/96/92/21.htm

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