摘要 :目的 探讨局部应用凝血酶特异性抑制剂(重组水蛭素)治疗脑出血后保护机制。 方法 成年雄性SD大鼠随机分为生理盐水组、脑出血组和水蛭素组(出血后3h)。以50μl自体血注入大鼠尾状核方法建立脑出血模型，应用干-湿重法观察脑水肿变化，前肢放置实验观察行为学。每组每时相点(1、2、3、7、14、28天)各6只大鼠。 结果 脑出血组和水蛭素组脑含水量与生理盐水组在24h、48h、72h比较P<0.05，7天时各组间无明显差异(P>0.05);脑出血组大鼠前肢放置实验结果与生理盐水组比较(P<0.05)，水蛭素组与脑出血组比较P<0.05。 结论 脑出血后24～48h水肿达高峰;重组水蛭素可明显减轻脑水肿，同时可以改善脑出血后的神经功能缺损。

    关键词 :脑出血;重组水蛭素;脑水肿

    Experimental study on local administration of recombinant hirudin for brain edema in rat models of intracerebral hemorrhage

    SHI Hong-juan，SHEN Xia，RONG Liang-qun，et al

    (Department of Neurology，Affiliated Hospital of Xuzhou Medical College，Xuzhou，Jiangsu221002，China)

    Abstract:Objective To investigate the protective effect and mechanism of local recombinant hirudin(thrombin inhibitor)on experimental intracerebral hemorrhage(ICH)in rats.Methods SD rats were randomly divided into saline control group，ICH control group and treatment group(with hirudin applied3hours after ICH).ICH model was established by injecting50μl of autologous blood into the caudate nucleus.At6time points(1d ......