Therapeutic effect of ANP on pulmonary microvascular endothelial cells injury induced by LPS

    LI Zhiª²Bin,LI Zhiª²Chao,YAN Zhiª²Qiang,SONG Jingª²Chun£¬ZHAO Pengª²Tao

    Department of Pathophysiology,School of Basic Medicine,Fourth Military Medical University,Xi¬ðan 710033,China

    ¡¾Abstract¡¿ AIM: To investigate the therapeutic effect of atrial natriuretic peptide (ANP) on pulmonary microvascular endothelial cells (PMVECs) injury induced by lipopolysaccharide (LPS). METHODS: Cultured PMVECs were divided into control group,LPS group and ANP treatment groups (LPS+ANP).The ANP treatment group was subdivided respectively into 3 groups treated with different concentrations of ANP (0ª±01,0ª±1 and 1 ¦Ìmol/L) and 4 groups treated for different time periods before adding ANP (0,6,12 and 24 h after LPS stimulation).The viability of PMVECs was examined by MTT assay and the cell cycle was analyzed by flow cytometry.The LDH activity and MDA production were measured from supernatants.RESULTS: ANP inhibited the decrease of the proportion of cells in S phase and G2ª²M phase induced by LPS.The A490 nm value declined and the LDH activity and MDA production increased in LPS groups compared with those in control groups (P<0ª±05). But the A490 nm value increased and the LDH activity and MDA production reduced in ANP treatment groups compared with those in LPS groups (P<0ª±05) in a doseª²andª²time dependent manner.CONCLUSION: ANP has some beneficial effects on LPSª²inuced PMVECs injury. ......