Early osteoblastic differentiation induced by dexamethasone promotes BMPª²2 transfected rMSCs osteoblast conversion

    LI Yan1,YAN Lu1,SHEN Min2

    1Department of Pharmacy,Center of Rehabilitation,2Department of Pharmacy,Xijing Hospital,Fourth Military Medical University,Xi¬ðan 710033,China

    ¡¾Abstract¡¿ AIM: To determine the effect of the induction of dexamethasone on the proliferation and differentiation phenotype of BMPª²2 modified rabbit mesenchymal stem cells (rMSCs).METHODS: The rMSCs,treated and untreated with dexamethasone,were transfected with BMPª²2 gene.The expression of BMPª²2 was determined by RTª²PCR and immunocytochemical staining.The changes of the proliferation and differentiation phenotype in the cells were observed by cell growth state,alkaline phosphatase (ALP) activity and osteocalcin (OCN) content.RESULTS: The expression of hBMPª²2 was confirmed in levels of mRNA and protein.Cell cultures of rMSCs pretreated with dexamethasone showed a higher ALP activity [(134ª±36¡À8ª±84) nkat/L in transfected group versus (104ª±02¡À7ª±83) nkat/L in control group] and higher osteocalcin content [(14ª±68¡À0ª±73) ¦Ìg/L in transfected group versus (6ª±52¡À1ª±21) ¦Ìg/L in control group].CONCLUSION: Early osteoblastic differentiation induced by dexamethasone can significantly promote BMPª²2 modified RMSCs osteoblast conversion. ......