Effect of nitric oxide on apoptosis caused by recurrent reversible myocardial ischemia/reperfusion injury

    YING Mingª²Zhong1,LI Xiaoª²Ying1,CHEN Xiao1,ZHAO Baoª²Lu2,ZHANG Deª²Liang2

    1Department of Geriatric,PLA General Hospital,Beijing 100853,China,2Biophysical Institute,Chinese Academy of Sciences,Beijing 100101,China

    ¡¾Abstract¡¿ AIM: To observe the relationship between the NO levels in the blood detected by electron spin resonance (ESR) and the myocardial apoptosis caused by the recurrent,reversible myocardial ischemia/reperfusion injury.METHODS: Fifteen New Zealand white rabbits were randomly divided into three groups (n=5): control group,Lª²Arg group,and Lª²NNA group.The rabbits were anesthetized with intravenous pentobarbital.A suture ligature was passed around the left anterior descending coronary artery (LAD) to have it snare occluded and reperfused.The LAD was occluded for 10 min three times.The first and second occlusions were followed by 10 min of reflow and after the third occlusion,the reperfusion was 120 min.RESULTS: Compared with those in control group at 5 min after the first reperfusion,NO and apoptosis levels (0ª±33¡À2ª±60) were elevated significantly in Lª²Arg group.In Lª²NNA group,NO and apoptosis level (0ª±13¡À1ª±30) decreased significantly.CONCLUSION: Elevated NO and apoptosis are involved in the process of myocardial ischemia/reperfusion injury. ......