癌症疫苗_____癌症疫苗；端粒酶逆转录酶；肿瘤；免疫疗法

构建携带hTERT I540PTD融合基因表达框的穿梭质粒

http://www.100md.com 《第四军医大学学报》 2005年第6期

癌症疫苗,,癌症疫苗；端粒酶逆转录酶；肿瘤；免疫疗法,构建携带hTERTI540PTD融合基因表达框的穿梭质粒,1材料和方法,2结果,3讨论,【参考文献】

     Construction of shuttle plasmids containing expression cassettes of hTERT I540PTD fusion genes

    GUO XiaoTong1，2，ZHANG JiRen1，LIU WenChao2，REN Jun2，LI LiWen3

    1Center of Oncoloy，Zhujiang Hospital，NanFang Medical University，Guangzhou 510282，China，2Department of Oncology，3PLA

    Institute of Orthopaedics,Xijing Hospital，Fourth Military Medical University，Xian 710033，China

    【Abstract】 AIM: To design and construct shuttle plasmids containing expression cassettes of hTERT I540PTD fusion genes.METHODS: The fusion genes were designed based on the preferred codons of mammalian and the amino acid sequences of hTERT I540 epitope,HIV1 TATPTD and PTD4,and were chemically synthesized in 6 oligonucleotides.The whole expression cassettes were then constructed by phosphorylation,annealing,and ligating into pSP72 in turns.After confirmed by restriction enzyme digestion and DNA sequencing,the fusion genes were subcloned into shuttle plasmid pDC 315.RESULTS: DNA sequencing results verified that the sequences of I540PTD4 and I540PTD were consistent with those we had designed.Both the pDC315I540PTD and pDC315I540PTD4 yielded a fragment of 112 bp following EcoRI digestion as we predicted.CONCLUSION: Shuttle plasmids containing expression cassettes of hTERT I540PTD and hTERT I540PTD4 have been successfully constructed,which provides a premise of the preparation of I540PTD DNA vaccines using adenoviruses as gene vector and for further investigation of the relationship between the transduction ability of PTDs and the levels of antigenspecific CTLs primed by the DNA vaccines. ......

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