戊四氮诱导发育鼠癫持续状态后海马突触素的变化及MK-801的影响
戊四,,发育鼠;癫持续状态;突触素;齿状回;MK-801,1材料和方法,2结果,3讨论,参考文献:
摘要 :目的 探讨戊四氮诱导发育鼠癫持续状态(SE)后海马结构的突触重建以及NMDA受体在其中的作用。 方法 利用图像分析仪测定戊四氮诱导的发育鼠癫持续状态模型及用NMDA受体拮抗剂MK-801治疗后海马结构内CA3区及齿状回内分子层突触素(p38)的阳性免疫反应产物的光密度值(A)。 结果 不同日龄SD大鼠SE后海马结构内各部p38阳性免疫反应产物光密度值较对照组显著增加，尤以CA3区苔藓纤维层和齿状回分子层内带为甚;MK-801可显著减少p38的表达。 结论 癫持续状态后存在突触重建现象，一方面是癫发作的结果，另一方面也可能是导致癫反复发作的分子学基础;在此过程中NMDA受体发挥着重要的作用。关键词 :发育鼠;癫持续状态;突触素;齿状回;MK-801
Change of synaptophysin in hippocampal formation of developing rat after pentylenetetrazol-induced status epilepticus and the effect of MK-801on it
CHEN Jing，YUAN Bao-qiang
(Dpartment of Pediatrics，Affiliated Hospital of Xuzhou Medical College，Xuzhou，Jiangsu221002，China)
Abstract:Objective To study the synaptic reorganization of hippocampal formation after pentylenetetrazol-induced sta-tus epilepticus in developing rats and the effect of NMDA receptor antagonist MK-801on it.Methods The optical density(A)of synaptophysin(p38)immunoreactive product was examined by image pattern analysis in the hippocampus of developing rats associated with pentylenetetrazol-induced status epilepticus and MK-801treatment.Results The A of p38positive im-munoreactive product in hippocmpal formation in the epileptic group was higher than that in the control ......
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