乳凝集素调节EEI-10细胞IL-2, IL-4及IFN-γ的分泌表达
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何振娟, 裴新红, 朱建幸, 杨凌云, 吴圣楣
乳凝集素;EEI-10;细胞因子,乳凝集素调节EEI-10细胞IL-2,IL-4及IFN-γ的分泌表达,何振娟,朱建幸,杨凌云,吴圣楣,裴新红,何振娟,通讯作者,Lactadherinadjustss
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何振娟, 朱建幸, 杨凌云, 吴圣楣, 上海交通大学医学院附属新华医院儿内科 上海市 200092
裴新红, Lindsley F. Kimball研究所细胞生化实验室 美国纽约 10021
何振娟, 1985年蚌埠医学院学士, 2001年上海第二医科大学(现上海交通大学医学院)博士, 副主任医师, 主要从事儿科黏膜免疫的研究.
国家自然科学基金资助项目, No. 30271375
通讯作者: 何振娟, 200092, 上海市控江路1665号, 上海交通大学医学院附属新华医院儿内科. hezhenjuan@gmail.com
电话: 021-65790000
收稿日期: 2006-02-22 接受日期: 2006-03-18
Lactadherin adjusts secretion and expression of interleukin-2, interleukin-4 and interferon-γ in EEI-10 cells
Zhen-Juan He, Xin-Hong Pei, Jian-Xing Zhu, Ling-Yun Yang, Sheng-Mei Wu
Zhen-Juan He, Jian-Xing Zhu, Ling-Yun Yang, Sheng-Mei Wu, Department of Pediatrics, the Affiliated Xinhua Hospital of Shanghai Jiao Tong University Medical School, Shanghai 200092, China
Xin-Hong Pei, Laboratory of Cell Biochemistry, Lindsley F. Kimball Research Institute, New York 10021, the United States
Supported by National Natural Science Foundation of China, No. 30271375
Correspondence to: Zhen-Juan He, Department of Pediatrics, Xin Hua Hospital, Shanghai Jiao Tong University Medical School, Shanghai 200092, China. hezhenjuan@gmail.com
Received: 2006-02-22 Accepted: 2006-03-18
Abstract
AIM: To define the function that lactadherin adjusts lymphocytes of gut-associated lymphoid tissue (GALT) cytokines secretion and expression.
METHODS: We extracted mRNA from human mammary cancer MCF-7 cells, and amplified the target gene from cDNA. The lactadherin was cloned into PET28 vector by digestion, ligation then transformation into DH5a cells to check positive clones. The cDNA encoding the desired polypeptide was transformed into BL-21 cells. The expression of recombinant protein was induced by isopropyl-β-D-thiogalactopyranoside (IPTG). The protein was purified with a nickel column. We detected the proliferation of EEI-10 cells after treated by different concentration of lactadherin using H3-Tdr assay ......
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