Stat5反义寡核苷酸联合5-氟尿嘧啶对胃癌细胞增殖与凋亡的影响
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马向涛, 余力伟, 王 杉, 杜如昱, 崔志荣
胃癌;信号转导通路;增殖;脱嗜作用,马向涛,余力伟,王杉,杜如昱,崔志荣,马向涛,通讯作者,EffectsofStat5antisen
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马向涛, 余力伟, 北京市海淀医院外科 北京市 100080
王杉, 杜如昱, 崔志荣, 北京大学人民医院外科肿瘤研究室 北京市 100044
马向涛, 2002年北京大学医学博士, 主治医师, 主要从事消化系统肿瘤的分子生物学研究.
国家自然科学基金资助项目, No. 30271269
通讯作者: 马向涛, 100080, 北京市海淀区中关村大街29号, 北京市海淀医院外科. xiangtao_ma@pku.org.cn
电话: 010-82619999-1887 传真：010-62653601
收稿日期: 2006-02-04 接受日期: 2006-03-20
Effects of Stat5 antisense oligonucleotide combined with 5-fluorouracil on proliferation and apoptosis of gastric cancer cells
Xiang-Tao Ma, Li-Wei Yu, Shan Wang, Ru-Yu Du, Zhi-Rong Cui
Xiang-Tao Ma, Li-Wei Yu, Department of Surgery, Beijing Haidian Hospital, Beijing 10080, China
Shan Wang, Ru-Yu Du, Zhi-Rong Cui, Department of Surgery and Division of Surgical Oncology, Peking University People’s Hospital, Beijing 100044, China
Supported by National Natural Science Foundation of China, No. 30271269
Correspondence to: Dr. Xiang-Tao Ma, Department of Surgery, Beijing Haidian Hospital, 29 Zhongguancun Avenue, Beijing 100080, China. xiangtao_ma@pku.org.cn
Received: 2006-02-04 Accepted: 2006-03-20
Abstract
AIM: To investigate the mechanism of Stat5 antisense oligonucleotide (Stat5 AS-ON) combined with 5-fluorouracil (5-FU) in the treatment of gastric cancer.
METHODS: Human gastric cancer cell line BGC823 was treated with Stat5 AS-ON and 5-FU, respectively, or in combination. The expression of Stat5, p-Stat5, cyclin D1 and Bcl-xL in the cells were detected by Western blot, and the cell cycle and apoptosis were detected by flow cytometry.
RESULTS: After treatment with Stat5 AS-ON and 5-FU for 72 h, the ratio of G1-phase cells was up-regulated from 65.7% to 78.2%, and that of S-phase cells was down-regulated from 18.6% to 10.5%; the percentage of apoptotic cells was increased from 7.4% to 21.6%. Stat5 AS-ON and 5-FU synergically inhibited the growth of gastric cancer cells, induced significant apoptosis of the cancer cells, and they reduced the expression and phosphorylation of Stat5, as well as the expression of cyclin D1 and Bcl-xL ......
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