吸烟者树突状细胞的功能状态研究分析
吸烟,,吸烟；树突状细胞；细胞因子；动脉粥样硬化,1对象与方法,2结果,【参考文献】
【摘要】 目的 研究吸烟者树突状细胞(DC)的功能。方法 分离吸烟者和非吸烟者外周血单个核细胞(PBMC)，在含粒细胞巨噬细胞集落刺激因子(GM-CSF)、白介素4(IL-4)的培养条件下制备DC。流式细胞仪检测DC表面CD86的表达；混合淋巴细胞反应(MLR)检测DC对T淋巴细胞的刺激能力；酶群免疫吸附法(ELISA)测定MLR上清液中细胞因子水平。结果 与非吸烟者比较，吸烟者DC表面CD86的表达明显增高，对T淋巴细胞的刺激能力增强；致炎细胞因子(IL-1β)分泌增多，抑炎因子(IL-10)分泌减少。结论 吸烟者DC明显激活，可能是吸烟致动脉粥样硬化的机制之一。【关键词】 吸烟；树突状细胞；细胞因子；动脉粥样硬化
Analysis of function of dendritic cells in smoking person
L Yong-heng，LI Zhi-liang，SHI Xiang-dong.Department of Cardiology，Zhujiang Hospital，Southern Medical University，Guangzhong 510287，China
【Abstract】 Objective To investigate the function of dendritic cells (DCs) in smoking person.Methods DCs were derived from human periphery blood monocyte cells (PBMCs) upon culture with interleukin-4 (IL-4) and granulocyte macrophage colony stimulating factor (GM-CSF) from smoking person.The surface co-stimulatory factor CD86 (7-2) of DCs were assessed by flow cytometry.Immunostimulatory capacity was measured by mixed lymphocyte reaction (MLR). ELISA was used to analyze the level of cytokines (IL-1β ......
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