侧向发育型肿瘤__(期刊论文)

大肠侧向发育型肿瘤细胞株双向电泳技术成功建立

http://www.100md.com 《第四军医大学学报》 2005年第7期

侧向发育型肿瘤,,侧向发育型肿瘤;细胞株；双向电泳；蛋白质组,1材料和方法,2结果,3讨论,【参考文献】

     Establishment of twodimensional gel electrophoresis in research of laterally spreading tumor cell line proteome

    TONG HuaSheng, ZHANG YaLi, JIANG Bo

    Institute for Digestive Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China

    【Abstract】 AIM: To establish twodimensional gel electrophoresis (2DE) in research of laterally spreading tumor (LST) cell line proteome. METHODS: Immobilized pH gradient (IPG) gels with different pH ranges were employed and a series of steps of 2DE were optimized. RESULTS: Satisfactory 2DE maps were obtained. 1356±43 spots were isolated when pH 3-10 line IPG gels were employed, and 1285±51 and 989 spots were isolated when pH 4-7 IPG gels with 250 μg and 150 μg protein loading were employed. CONCLUSION: With optimization of the experimental conditions, 2DE of LST cell line has been successfully established.

    【Keywords】 LST; cell line; twodimensional gel electrophoresis; proteome

    【摘要】目的：建立侧向发育型肿瘤细胞(LST)株双向电泳技术. 方法：提取LST细胞株总蛋白质 ......

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