噬菌体随机肽库__(期刊论文)

抗hTNFα中和抗体识别表位的筛选和鉴定

http://www.100md.com 《第四军医大学学报》 2005年第7期

噬菌体随机肽库,,肿瘤坏死因子；噬菌体随机肽库；抗体；表位,1材料和方法,2结果,3讨论,【参考文献】

     Screening and identification of antihTNFα monoclonal antibody recognized hTNFα epitopes from 7TMphage display peptide library

    ZHANG Cun, ZHANG YingQi, YAN Zhen

    Biotechnology Center, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To screen out short peptide epitopes from 7TMphage display peptide library, which are capable of combining with antihTNFα monoclonal antibody. METHODS: The epitopes of human tumor necrosis factor were screened from 7TMphage display peptide library by using antihTNFα antibody as target protein and identified by sandwich ELISA and dotblot. RESULTS: After 3 rounds of screening, 15 of the 50 phage clones were identified as positive clones, which bound to the antihTNFα antibody. The amino acid sequences of the positive clones were WTFKMQP, WPFKMSP, WPYKMIP and WNYKMLP. CONCLUSION: The framework of the screened epitopes is similar and the epitopes can specifically bind to the antihTNFα antibody, which provides the basis for the development of short peptide vaccines. ......

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