Relationship between expression of Stathmin gene in cancer cells and its sensitivity to chemotherapeutic drugs

    JING Xiaoª²Rong, LIU Li, ZHAO Hui, ZHANG Huiª²Zhong

    Department of Clinical Diagnosis, Tangdu Hospital, Fourth Military Medical University, Xi¬ðan 710038, China

    ¡¾Abstract¡¿ AIM: To investigate the sensitivity of eight kinds of cancer cells to two kinds of chemotherapeutic drugs: Vincristine and Docetaxel and to study the relationship between the sensitivity and the expression of Stathmin gene in these cells. METHODS: The chemosensitivity of the eight kinds of cancer cells to Vincristine (VCR) and Docetaxel (DOC) was detected by MTT colorimetric assay and morphological observation. Real time PCR technology was used to examine the expression of Stathmin gene in these cells. RESULTS: The inhibition rates of the eight kinds of cancer cells by VCR and DOC were respectively 58% and 48% for human osteosarcoma cells line Sospª²9607 (9607), 56% and 44% for human osteosarcoma cells line Sospª²9901 (9901), 37% and 46% for cervical cancer cells line Hela (Hela), 34% and 12% for human hepatocellular carcinoma cells line HHCC (HHCC), 31% and 31% for lung cancer cells line A549 (A549), 17% and 34% for largngoª²carcinoma cells line Hepª²2 (Hepª²2), 16% and 15% for gastric cancer cells line MKN45 (MKN45), and 15% and 3% for human hepatocelluar carcinoma cells line 7721 (7721) cells. The intensity of the expression of Stathmin gene in the eight kinds of cells was different and was correlated with the sensitivity mentioned above. CONCLUSION: The expression of Stathmin gene in cancer cells is related to its sensitivity to the chemotherapeutic drugs. The detection of the expression of Stathmin gene in tumor cells may be of some clinical significance in choosing a chemotherapy agent. ......