PTA1_____PTA1;_启动子;_RACE;

AP1对人CD226　PTA1启动子的调控作用

http://www.100md.com 《第四军医大学学报》 2005年第9期

PTA1,,PTA1;,启动子;,RACE;,AP1,AP1对人CD226PTA1启动子的调控作用,1材料和方法,2结果,3讨论,【参考文献】

     Regulation of PTA1 promoter by AP1

    JIAN JinLong, CHEN LiJie, CAO YunXin, OUYANG WeiMing, JIN BoQuan

    Department of Immunology, School of Basic Medicine, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To identify the transcription start site of human PTA1 gene and to investigate the regulation of PTA1 promoters by AP1. METHODS: The transcription start site was cloned by 5′RACE method. The AP1 expression vector and the reporter vector containing PTA1 promoter were cotransfected into HHCC cell line. The luciferase activity of transfected HHCC was determined after 48 h culture. RESULTS: The transcription start site was located at the 229 bp upstream of ATG. AP1 upregulated PTA1 promoter P1 and P2, while the transcription factor ets1 had different regulatory effects on PTA1 promoter P1 and P2 when cotransfected with AP1. CONCLUSION: The transcription start site of PTA1 is at the 229 bp upstream of ATG and the PTA1 promoters P1 and P2 can be regulated by AP1 and ets1. ......

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