Preparation of rhBMPª²2ª²loaded gelatin microspheres and biological effects of rhBMPª²2ª²GMs on proliferation and alkaline phosphatase expression of human periodontal ligament cells

    XIAO Yuª²Hong1, WU Hong2, LIU Hongª²Wei1, CHEN Shuª²Jun1, LI Fei3, WU Daoª²Cheng2

    1Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China, 2Department of

    Chemistry, School of Pharmacy, 3Department of Prosthodontics, College of Stomatology, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To evaluate the biological effects of recombinant human bone morphogenetic proteinª²2 gelatin microspheres (rhBMPª²2ª²GMs) on the proliferation and alkaline phosphatase (ALP) expression of human periodontal ligament cells (PDLCs). METHODS: MTT method and enzyme dynamics method were used to observe the proliferation and ALP expression of PDLCs after different doses of rhBMPª²2 and rhBMPª²2ª²GMs were added into the culture medium. RESULTS: Both rhBMPª²2 and rhBMPª²2ª²GMs promoted the proliferation and increase the ALP activity of PDLCs in a concentrationª²related manner. Significantly greater proliferation and expression were found in rhBMPª²2ª²GMs group compared with those in rhBMPª²2 group. CONCLUSION: The results indicate that rhBMPª²2ª²GMs can significantly promote the proliferation and increase the ALP activity of PDLCs by sustained release. ......