乙型肝炎病毒_____乙型肝炎病毒；转录体；血清；母婴传播

乙肝病毒转录体检测在母婴传播中的应用

http://www.100md.com 《疑难病杂志》 2006年第2期

乙型肝炎病毒,,乙型肝炎病毒；转录体；血清；母婴传播,1资料和方法,2结果,3讨论,参考文献

     【摘要】目的探讨乙型肝炎病毒(HBV)转录体的检测在母婴传播中的意义。方法自母亲及其婴儿血清中提取核酸，经PCR及RTPCR分别扩增HBV DNA和RNA，琼脂糖凝胶电泳显示产物，Southernblotting验证反应的特异性，取代表性产物克隆、测序。结果 HBeAg(+)母亲的XDNA的表达(75.0%)和fRNA的表达(65.0%)显著高于HBeAg(-)的母亲(分别为37.5%和20.8%，P<0.05和P<0.005)；所有表达fRNA的母亲也同时表达trRNA，在20例XDNA(-)、fRNA(-)的母亲中，仍有10例可检测到trRNA。新生儿XDNA的表达(4.6%)和fRNA的表达(6.8%)较其母亲显著降低；占优势表达的是trRNA(52.3%)，且与其母亲trRNA的表达状态密切相关。母亲为trRNA(+)的新生儿trRNA的表达显著高于母亲为trRNA(-)者。15例HBsAb(+)的新生儿均未检出XDNA和fRNA，但11例仍可检测到trRNA。结论与HBV DNA及其表达产物相比，trRNA在HBV母婴垂直传播过程中有可能是一个出现更早期的可检测指标，它有助于更精确的确定新生儿HBV感染的状态。

    【关键词】乙型肝炎病毒；转录体；血清；母婴传播

    The significance of hepatitis B virus transcripts in transmitting from mother to infant

    GUO Yong,MA Zhenzhi,XIU Xia,et al.

    Weifang Health Institute for Women and Children,Shandong,Weifang 261011,China

    【Abstract】 Objective To investigate the clinical significance of hepatitis B virus (HBV) transcripts in transmitting from mother to infant.Methods The serum viral nucleic acids extracted from both mother and infant were analyzed using PCR and RTPCR targeted to the HBV X proteinencoding region.Reaction specificity was confirmed by Southern blotting.Representative amplification products were cloned and sequenced.Results The expression of XDNA (75.0%) and fRNA (65.0%) in HBeAg(+) mothers was significantly higher than that in HBeAg(-) mothers (P<0.05 and P<0.005 respectively).All the mothers expressing fRNA expressed XDNA and trRNA at the same time.10 out of 20 mothers were trRNA(+),who were both XDNA(-) and fRNA(-).The detective rate of XDNA and fRNA in infants (4.6% and 6.8% respectively) was significantly lower than that in their mothers,whereas the detective rate of trRNA was high (52.3%),which was expressed superiorly in infants serum.Infants' trRNA expression was associated closely with their mothers,and the trRNA expressed in infants from trRNA(+) mothers was higher than those whose mother were trRNA(-).In 15 HBsAb(+) infants who were both XDNA(-) and fRNA(-),11 still could be detected for trRNA.Conclusion As compared with HBV DNA and HBV gene expression products,trRNA might be a earlier detectable serum maker for identifying HBV infection profile accurately in infants,which is associated closely with their mothers' expression of trRNA. ......

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