肝星状细胞__(期刊论文)

JNK信号通路在乙醛刺激的肝星状细胞凋亡中的作用

http://www.100md.com 《第四军医大学学报》 2005年第10期

肝星状细胞,,肝硬化；肝星状细胞；乙醛；细胞凋亡；cJun氨基末端激酶；SP600125,1材料和方法,2结果,3讨论,【参考文献】

     Effects of JNK signaling pathway on apoptosis in rat hepatic stellate cells stimulated by acetaldehyde

    JIANG MingDe，ZHONG XianFei，MA HongDe，ZENG WeiZheng,LI XiaoAn

    Department of Gastroenterology，General Hospital of Chengdu Military Command,Chengdu 610083,China

    【Abstract】 AIM: To study the effects of SP600125,a specific blocking agent of cJun Nterminal kinase (JNK),on the apoptosis in rat hepatic stellate cells (HSC).METHODS: Rat hepatic stellate cells stimulated by acetaldehyde were incubated with different concentrations of SP600125 in vitro.The changes of apoptosis were analyzed by agarose gel eletrophoresis of DNA fragmentation and flow cytometry and the activity of JNK was measured by Western blot.RESULTS: SP600125 at different concentrations (20 ng/mL,60 ng/mL and 100 ng/mL) not only suppressed the activity of JNK [MVI: (356±09),(244±11),(34±02) vs (482±09),P<005],but also increased apoptosis of HSC [(111±04)%,(269±09)%,(331±13)% vs (60±05)%,P<005].CONCLUSION: The inhibitory effect of SP600125 on JNK binding activity may be involved in the apoptosis of HSC. ......

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