Effects of autologous satellite cells transmplantation on cardiac function in acute myocardial infraction

    XU Qingª²Bin1, ZHANG Fuª²Min1, YANG Zhiª²Jian1, CHEN Yiª²Jiang1, GAO Xiang2, QIAN Yi1, MA Wenª²Zhu1

    1Department of Cardiology, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029 China, 2Model Animal Research Center, Nanjing University, Nanjing 210034, China

    ¡¾Abstract¡¿ AIM: To study the effects of autologous skeleton muscle satellite cells (SC) transplantation on cardiac function in acute myocardial infraction (AMI). METHODS: The proximal potion of left anterior descending coronary artery (LAD) was ligated in young pigs to establish the AMI model and the young pigs were as donors and recipients of autologous SC transplantation. In treatment group, SC were isolated from skeleton muscle, cultureª²expanded and labeled with DAPI. The SC were implanted into AMI area through intramyocardium. In control group (n=5), the same volume of cell culture medium was implanted into AMI area through intramyocardium. Gate cardiac blood pool was performed at the end of 1 and 4 weeks after AMI, respectively. The cardiac specimens were harvested for pathological examination at the end of 4 weeks. RESULTS: SC were spindleª²shaped and adhered on the bottom of the flask after 48ª²hour culture. If calf bovine serum concentration decreased to 20 mL¡¤L-1, SC commenced the fusion process, which resulted in the formation of multinucleated myotubes. New islands and striated muscles were found in treatment group at the site of implantation. The new muscles cell didn't show histological evidence of intercalated discs and centrally located nuclei. Left ventricular ejection fraction (LVEF) increased both in treatment group and control group, but LVEF was higher in treatment group than that in control group [(40¡À2)% vs (33¡À2)%, P<0.01], and 1/3 filling fraction were greater in treatment group than that in control group (0.60¡À0.07 vs 0.54¡À0.29, P<0.05). CONCLUSION: SC can differentiate into striated muscles under cardiac microenvironment and SC transplantation can improve both systolic and diastolic function of infracted heart. ......