脊髓损伤_____基质金属蛋白酶9；脊髓损伤；细胞

脊髓损伤后MMP9表达的时空分布及其意义

http://www.100md.com 《创伤外科杂志》 2005年第3期

脊髓损伤,,基质金属蛋白酶9；脊髓损伤；细胞,材料与方法,1动物分组和模型制作,2MMP9的免疫组化检测,3图像分析,4统计学处理,1急性损伤脊髓

     摘要：目的通过研究大鼠急性脊髓损伤后基质金属蛋白酶9(MMP9)表达的时空变化，探讨基质金属蛋白酶9在急性脊髓损伤后的作用。方法使用改良Allen法制作大鼠急性脊髓损伤模型，检测脊髓损伤后基质金属蛋白酶9在损伤脊髓中表达的时空分布特点。结果损伤组急性脊髓损伤后1小时，可见基质金属蛋白酶9的表达，损伤后1～2天达到高峰，7天仍有表达。阳性细胞主要表达在缺血神经元、血管内皮细胞和嗜中性粒细胞。对照组未见基质金属蛋白酶9表达。结论基质金属蛋白酶9在脊髓损伤后的早期表达，提示基质金属蛋白酶9可能参与脊髓损伤后水肿形成和炎性细胞的浸润，加重了继发性脊髓损伤。

    关键词：基质金属蛋白酶9；脊髓损伤；细胞

    Expression of matrix metalloproteinase9 after acute spinal cord injury

    ZHOU Hua,LIU Hua

    (Department of Traumatology,First Affiliated Hospital of Suzhou University,Suzhou 215006,China)

    Abstract：Objective To investigate the early expression of matrix metalloproteinase9 after actue spinal cord injury.Methods The models of spinal cord injury in rats were induced with Allen's way and the expression of matrix metalloproteinase9 in injuried spinal cord was observed.Results The expression of matrix metalloproteinase9 increased 6h after spinal cord injury，which reached its peak 1,2 days after spinal cord injury(SCI).The matrix metalloproteinase9 positive cells were mainly expressed on ischemia neuron, angioendothelial cells and neutrophile.The express of matrix metalloproteinase9 wasn't found in the shamoperated rats.Conclusion The early matrix metalloproteinase9 may be partially involved in the secondary pathogenesis of spinal cord injury. ......

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