摘要: 目的 建立实验性蛛网膜下腔出血后内皮细胞损伤模型，并观察血性脑脊液(bloody cerebralspi-nal fluid，BCSF)对培养的微血管内皮细胞的损害作用。 方法 将体外培养的兔脑微血管内皮细胞分为对照组和BCSF刺激组，通过MTT比色法选择恰当孵育时间的BCSF作为刺激因素，观察病理形态学改变、细胞计数、流式细胞术细胞周期，分析评估内皮细胞损害和增殖状况。 结果 与对照组比较，孵育7天BCSF可使内皮细胞出现明显的病理形态学改变，贴壁细胞数量减少，MTT吸光度下降(P<0.01)，G 0 ～G 1 期细胞比例增高(P<0.01)。 结论 BCSF可直接造成明显的兔脑微血管内皮细胞病理形态学损害，并抑制血管内皮细胞代谢和增殖。

    关键词: 脑血管痉挛;蛛网膜下腔出血;内皮细胞;兔

    Effect of experimental subarachnoid hemorrhage on microvessel endothelial cells of brain

    CHEN Zhi，WUNan，LI Fei，et al.

    (Departments of Neurosurgery，Southwest Hospital，Third Military Medical University，Chongqing 400038，China)

    Abstract: Objective To establish an endothelial cell model in vitro following experimental subarachnoid hemor-rhage，and study the cytoxic effect of bloody cerebrospinal fluid(BCSF)on endothelial cells.Methods Rabbit brain mi-crovessel endothelial cells(BmvECs)were divided into a control group and a BCSF group.MTT assay was taken to evalu-ate the injury of BCSF incubated for different time on BmvECs in order to decide the suitable BCSF as pathogen.Morpho-logical changes，cell counting ......