HCCª²X gene expression in hepatocarcinoma and NMR1H spectrum characteristics of correlative marker proteins

    HU Juan1, WEI Min1, ZHANG Mingª²Shan2, TAN Yingª²Jun2, LI Yong2, WANG Yu2, ZENG Huaª²Song3, JING Xiuª²Jing3, YANG Jiª²Fei3, HU Zongª²Hai2

    1Teaching & Research Section of Military Medicine, Chengdu Medical College, Third Military Medical University, Chengdu, 610083, China, 2Department of Obstetrics and Gynecology, Chengdu Military General Hospital, Chengdu 610083, China, 3Department of Clinical Laboratory, Chengdu Hospital, Chinese People¡¯s Armed Police Forces, Chengdu 610041, China

    ¡¾Abstract¡¿ AIM: To obtain the information expressed by HCCª²X mRNA in normal liver, parahepatic tissue and hepatocarcinoma with an information probe, catch the correlative protein spectrum characteristics of nonª²carcinomatous occupancy by the analytical technique of NMR1H spectra, and to discuss their rule and clinical value. METHODS: The obtained gene was labeled as a probe with u-12Pª²DATP.The MTN blot diaphragm was hybridized with 32Pª²HCCª²X and the function of genetic expression was detected with western blot. Parameter of a chemical shift of 19 kinds of monomeric amino acid (aa)was analyzed by the NMR1H spectrum apparatus. The Hand Book of Protonª²NMR Spectra and Data was taken as the reference and analytic conditions were set up. RESULTS: The external clone formation experiment of BEL7402 transfected hepatocarcinoma cells showed that HCCª²X had a distinct inhibitory effect on the growth of them in vivo with low expression or without expression in hepatocarcinoma tissue and a significant difference as compared with normal tissue (P<0.01); and of the 19 kinds of monomer aa analyzed by NMR1H spectra, 14 were totally accorded with the spectra, 1 was similar, and Trp and His were obtained for the first time respectively. CONCLUSION: HCCª²X may be the pathogenic gene of hepatocarcinoma and highly homologous HFARP has an inhibitory effect on vascular endothelial cell apoptosis; The conditions of analyzing the correlative marker protein with NMR1H spectra can meet the demands of catching the information from the fingerprint region and provide a new method for the correlative study of physiopathologic mechanism. ......