感染幽门螺杆菌小鼠肝组织Cyclin D1和PCNA的表达
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田雪飞, 范学工, 黄 燕, 张 艳, 朱 才
幽门螺杆菌;肝细胞癌;PCNA;CyclinD1;小鼠田雪飞,范学工,黄燕,张艳,朱才.感染幽门螺杆菌小鼠肝组织CyclinD1和PCNA的表达. 世界华人消化杂志2006;14(14)1
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田雪飞, 范学工, 黄燕, 张艳, 朱才, 中南大学湘雅医院传染科 湖南省长沙市 410008
田雪飞, 2001年湖南中医药大学博士, 2002年中南大学湘雅医院博士后流动站, 副教授, 主要从事幽门螺杆菌方面的研究.国家自然科学基金资助项目, No. 30271171
国家教育部骨干教师基金资助项目, No. 教技司2000-65
湖南省自然科学基金资助项目, No. 01jjy2114
湖南省卫生厅科研基金资助项目, No. 00022
通讯作者: 范学工, 410008, 湖南省长沙市湘雅路87号, 中南大学湘雅医院传染科. xgfan@hotmail.com
电话: 0731-4327392 传真: 0731-4327332
收稿日期: 2005-11-15 接受日期: 2005-11-26
Expression of Cyclin D1, proliferating cell nuclear antigen in liver of C57BL/6 mice infected with Helicobacter pylori
Xue-Fei Tian, Xue-Gong Fan, Yan Huang, Yan Zhang, Cai Zhu
Xue-Fei Tian, Xue-Gong Fan, Yan Huang, Yan Zhang, Cai Zhu, Deparment of Infectious Diseases, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China
Supported by National Natural Science Foundation of China, No. 30271171; Teacher Foundation from the National Ministry of Education, No. 2000-65; the Natural Science Foundation of Hunan Province, No. 01jjy2114; and the Scientific Research Foundation from Health Bureau of Hunan Province, No. 00022
Correspondence to: Dr. Xue-Gong Fan, Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China. xgfan@hotmail.com
Received: 2005-11-15 Accepted: 2005-11-26
Abstract
AIM: To detect the expression of Cyclin D1 and proliferating cell nuclear antigen (PCNA) in liver tissues of C57BL/6 mice infected with H pylori by oral inoculation.
METHODS: Thirty C57BL/6 strain mice, used as experiment animal, were orally inoculated with H pylori SS1 strain and fed in laminar flow cabinets for 8 mo. H pylori 16S rRNA in liver was examined by nested polymerase chain reaction (PCR), and then mRNA and protein were extracted from the positive liver tissues. The mRNA and protein expression of Cyclin D1 and PCNA were detected by reverse transcription PCR (RT-PCR) and Western blot, respectively ......
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