新生儿CYP1A1、GSTM1、GSTT1基因的多态性分析
婴儿,,婴儿，新生；细胞色素P450；谷胱甘肽-S-转移酶；基因,1材料与方法,2结果,3讨论,【参考文献】
【摘要】 目的 研究新生儿CYP1A1、GSTM1、GSTT1基因的多态性分布，为新生儿建立相应基因型记录，达到防病治病的目的。方法 收集新生儿脐血，抽提其中有核细胞的DNA，PCR扩增CYP1A1、GSTM1、GSTT1基因的特征性外显子片段。限制酶切CYP1A1扩增产物，RFLP分析每个标本的基因型；非变性聚丙烯酰胺凝胶电泳分析GSTM1、GSTT1的基因型。结果 CYP1A1、GSTM1和GSTT1基因型均呈多态性分布，CYP1A1的基因型分布有3种，分别为A/A基因型(占60.9%)、A/G基因型(占34.5%)、G/G基因型(占4.5%)；GSTM1的基因型有2种分别为GSTM1+/+和GSTM1 +/0(占85.5%)、GSTM10/0(占14.5%)；GSTT1基因型分布为GSTT1+/+和GSTT1 +/0(占76.4%)，GSTT1 0/0(占23.6%)。结论 在正常出生的新生儿中，代谢酶CYP1A1、GSTM1、GSTT1基因存在着多态性分布的现象。【关键词】 婴儿，新生；细胞色素P450；谷胱甘肽-S-转移酶；基因
Distribution of polymorphism of infant CYP1A1，GSTM1 and GSTT1 genotypes
ZHOU Yi,XIONG Mi,MA Zhiming,et al.
Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China
【Abstract】 Objective To establish the genotype records for the new born infants to guide prevention and therapy of disease by investigating distribution of polymorphism of infant CYP1A1,GSTM1 and GSTT1 genotype.Methods The DNA was extracted from new born infants cord blood.The exon 4 of GSTM1 gene,specific region of GSTT1 gene and exon 7 of CYP1A1 gene were amplified by PCR,the polymorphism of CYP1A1 gene were analyzed by PCRRFLP,the polymorphism of GSTM1 and GSTT1 gene were detected by gel eletrophoresis.Results The genotype of CYP1A1,GSTM1,GSTT1 were polymorphic distribution in infants.There were three genotype of CYP1A1 gene according to the 4889 base A or G in exon 7 region,and A/A ratio was 60.9%,A/G ratio was 34.5%.G/G ratio was 4.5%.The two genotype of GSTM1 was GSTM1+/+(including GSTM1 +/0)and GSTM1 0/0 based on the amplified alleles deleted or not,their ratio were 85.5% and 14.5%.The genotype ratio of GSTT1 were GSTT1+/+(including GSTT +/0)76.4% and GSTT1 0/0 23.6%.Conclusion There are polymorphic distribution of infant metabolic enzyme CYP1A1,GSTM1,GSTT1.It indicates that the varability of suffering disease may be among the individuals. ......
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