【摘要】 目的 探讨钝顶螺旋藻多糖(PSP)对HSV-2糖蛋白gB mRNA表达的影响及其机制，为进一步开发该药提供理论依据。方法 以不同剂量的PSP作用于HSV-2感染的Vero细胞，应用地高辛标记寡核苷酸探针，检测HSV-2糖蛋白gB mRNA的表达。结果 PSP作用组HSV-2糖蛋白gB mRNA的表达显著低于阳性对照组(P<0.01)。结论 PSP抗HSV-2作用的机制之一为抑制HSV-2糖蛋白gB基因的转录。

    【关键词】 螺旋藻多糖；单纯疱疹病毒2型；原位杂交

    Inhibition of HSV-2 glycoprotein gB mRNA expression by polysaccharide from spirulina platensis(PSP)

    ZHOU Xiao-dong,

    YU Hong.Qingdao Stomatological Hospital, Qingdao 266003,China

    【Abstract】 Objective To explore the effects and mechanism of PSP on the expression of HSV-2 gB mRNA and to provide a theoretical support for developing new drugs.Methods PSP was applied at various concentrations to Vero cells infected by HSV-2, In situ hybrization was adopted to explore the expression of HSV-2 gB mRNA with digoxigenin(DIG)-labeled HSV-2 oligoprobe. Results HSV-2 gB mRNA were less expressed in PSP treated group than that in the control (P<0.01).Conclusion One of the mechanism of PSP against HSV-2 may be explained by the considerable inhibition of HSV-2 gB gene trscription. ......