【摘要】 目的 探讨去甲肾上腺素对人THP-1源性巨噬细胞SR-A1表达的影响。方法 不同浓度的去甲肾上腺素(10pmol/L～10 μmol/L)作用THP-1源性巨噬细胞24h，运用逆转录多聚酶链反应检测其SR-A1 mRNA的表达。Western 印迹检测SR-A1蛋白的表达。结果 1 μmol/L及10 μmol/L的去甲肾上腺素作用THP-1源性巨噬细胞后，其SR-A1 mRNA和蛋白水平上升(P<0.05)。结论 应激状态的去甲肾上腺素能升高THP-1源性巨噬细胞SR-A1基因的表达。

    【关键词】 去甲肾上腺素； THP-1源性巨噬细胞； SR-A1

    Effects of noradrenaline on SR-A1 expression in human THP-1 macrophage cells

    CHEN Guang-Jun， GUO Xiao-Fang， XIAO Rong,et al.

    Emergency Medical Center，Second People’s Hospital of Yueyang, Hunan 414000,China

    【Abstract】 Objective To investigate the effect of noradrenaline on the expression of SR-A1 in human THP-1 macrophage cells.Methods We use the different concentrations of noradrenalin(10 pmol/L～10 μmol/L)which incubate with THP-1 macrophage cells 24 hours. then the mRNA levels of SR-A1 were determined by reverse transcription polymerase chain reaction (RT-PCR). Western-blot analysis were used to dectect protein level of SR-A1.Results THP-1 macrophage cells is treated with 1μmol/L and 10 μmol/L noradrenalin, the mRNA and protein levels of SR-A1 increase(P<0.05).Conclusion Stress concentrations of noradrenalin cause SR-A1 expression increased in human THP-1 macrophage cells. ......